Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice

Metabolic alterations are a hallmark of cancer. While determining metabolic changes in vitro has delivered valuable insight into the metabolism of cancer cells, it emerges that determining the in vivo metabolism adds an additional layer of information. Here, we therefore describe how to measure the in vivo metabolism of cancer tissue using ¹³C glucose infusions in mice.