Effects of the Serum Containing Aspongopus chinensis Dallas on the Expression of SW480 Apoptosis-associated Factors,FADD and p53

[Objective] The aim was to study the effects of the serum containing Aspongopus chinensis Dallas on proliferation of SW480(Human colon adenocarcinoma cell line) cells and expression of apoptosis-associated factors,Fas-associated death domain protein(FADD) and p53.[Methods] 20 male SD rats were randomly and equally divided into two groups of control group and Aspongopus chinensis Dallas-containing serum group,the rats in the serum group were administrated intragastrically with A.chinensis Dallas for 5 d and then the blood was collected to prepare serum containing A.chinensis Dallas,drug-containing serum was added to culture SW480 cells.Proliferation of SW480 cells was detected by cell proliferation test with CCK8.The flow cytometry was used to assay the apoptosis.The content of p53 and FADD were determined by western blot and the content of p53 mRNA and FADD mRNA by fluorescence quantitative PCR.[Results] The serum containing A.chinensis had an obvious inhibitory effect on the proliferation of SW480 for 72 hours.Drug-containing serum showed positive effect on cell apoptosis.The rate of apoptosis was higher than the control(P0.01).The results of Western bolt and FQ PCR indicated that the Aspongopus chinensis Dallas-containing serum could intensify the FAAD expression and decrease the expression of p53.[Conclusion] The serum containing A.chinensis Dallas had a significant inhibitory effect on the proliferation of SW480.The mechanism of inducing SW480 cells apoptosis might be relevant to stimulate FADD and suppress p53.