Optimization of Culture Condition for Bacillus licheniformis 1.934 Production

Bacillus licheniformis is very important as a soil microorganism to increase the availability and uptake of mineral nutrients for plants.In this study,the effect of culture condition on Bacillus licheniformis 1.934 production was investigated.On the basis of single factor experiments,a central composite design was used to optimize the prime factors.The results were analyzed by response surface.Analysis results show that the optimal culture condition is as follows:temperature 35℃,flask shaking speed 150r/min,amount of inoculation 3.6%,pH 7.5.Bacillus licheniformis 1.934 grew well under this condition,incubated for 20h,the highest amylase activity(12.7U /mL) can be obtained;incubated for 27h,the highest cell number can be gotten.