Objective To understand the variations of tendency of drug-resistance,the origin and the distributing of Acinetobacter baumannii and provide basis for selection of clinical drugs. Methods The data concerning Acinetobacter baumannii infection in our hospital in 2001~2005 were retrospectively and statistically analyzed. Results The total strains of Acinetobacter baumannii isolated from this hospital was increased from 12 strains in 2001 to 63 strains in 2005 ,and a panresistant strain was detected in the last five years. High drug-resistance and pan-resistant strains were accounted for 15.6%. Acinetobacter baumannii was most sensitive to imipenem. 60% of Acinetobacter baumannii were isolated from sputum specimens. Conclusion The infection rate of Acinetobacter baumannii is increasing and measures be adopted for control Acinetobacter baumannii infection in hospital.