DESIGN AND CHARACTERISTICS OF MONOCLONAL ANTI-BODIES SPECIFIC TO RESPIRATORY SYNCYTIAL VIRUS

An antibody panel that includes 6 MCA specifically interacting in ELISA with RSV strains of both existing antigenic gro-ups (A and B) has been designed. The new MCA are directed to 6 different conformation-dependent F protein epitopes, 3 of them inducing the synthesis of virus-neutralizing antibodies. Using real-time PCR with reverse transcription, immunoblotting, identification of MCA virus-neutralizing activity, competitive ELISA and cell-ELISA, it was shown that only the MCA who se synthesis was induced by variable RSV F protein determinants (unlike MCA directed to conservative determinants that are common for the viruses of various antigenic groups) have the virus-neutralizing activity. Differences in the interaction between RSV, MCA 4F2 and MCA 5F3 make it possible to identify by cell-ELISA of antigen group (RSV-A or RSV-B) of strains isolated from clinical samples.