拟南芥
生物
拟南芥
基因
苗木
选择(遗传算法)
作者
Marta Bjornson,Xingshun Song,Abhaya M. Dandekar,Annaliese K. Franz,Georgia Drakakaki,Katayoon Dehesh
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2015-07-05
卷期号:5 (13)
被引量:2
标识
DOI:10.21769/bioprotoc.1519
摘要
Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality.
This protocol describes a chemical genetic screen using Arabidopsis thaliana seedlings, which has led to recognition of novel players in the plant general stress response.
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