Microinjection into the gonads of C. elegans using Eppendorf InjectMan ® 4

Caenorhabditis elegans is a well-established model organism for a wide variety of research fields. The simple and highly conserved structure, together with its short generation time and transparent body, renders C. elegans a perfect experimental system for genetic alterations. Microinjection is an established method for generating transgenic C. elegans strains. In this Application Note we describe the microinjection procedure using the Eppendorf InjectMan 4 micromanipulator.