老茧
器官发生
葛根
开枪
Murashige和Skoog培养基
生物
外植体培养
植物
园艺
医学
生物化学
基因
病理
体外
替代医学
作者
T K Bindu,P. Sheema Dharmapal,P. S. Udayan
出处
期刊:International Journal of Environment, Agriculture and Biotechnology
[AI Publications]
日期:2018-01-01
卷期号:3 (2): 589-593
被引量:2
标识
DOI:10.22161/ijeab/3.2.37
摘要
Pueraria tuberosa (Roxb.ex Willd.)DC. is a perennial woody climber, commonly known as Indian Kudzu in English, Vidarikand in Hindi and Vidari in Sanskrit.The tubers are used in different systems of medicine viz.Ayurveda, Folk, Homoeopathy, Siddha, Tibetan and Unani.The present study aims to develop an effective protocol for optimum callus induction and organogenesis in Pueraria tuberosa.Callus cultures were first established by inoculating tender leaf explants in Murashige and Skoog's (MS) medium supplemented with different concentrations of 6-Benzylaminopurine ( 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg.L-1) with 1-Naphthaleneacetic acid (0.5 mg.L-1).Maximum callus induction and shoot organogenesis was observed in MS medium containing 1.5 mg.L-1 BAP with NAA 0.5mg.L-1.Fresh weight of the organogenic greenish granular hard callus was 4.70±0.10gm.Shoot organogenesis was observed after 65 days of culture.Maximum shoot buds developed from callus on MS media augmented with 1.5 mg.L-1 BAP with 0.5 mg.L -1 NAA.
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