突变体
根瘤
生物
基因
莲藕
结核(地质)
基因敲除
侧根
根瘤菌
根瘤菌
遗传学
细胞生物学
共生
拟南芥
细菌
古生物学
作者
Daisuke Uchida,Shimpei Magori,Takashi Soyano,Satoru Okamoto,Chie Yoshida,Koji Yano,Shusei Sato,Satoshi Tabata,Katsushi Yamaguchi,Shuji Shigenobu,Naoya Takeda,Takuya Suzaki,Masayoshi Kawaguchi
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2013-04-01
卷期号:54 (4): 433-447
被引量:94
摘要
The interaction of legumes with N2-fixing bacteria collectively called rhizobia results in root nodule development. The number of nodules formed is tightly restricted through the systemic negative feedback control by the host called autoregulation of nodulation (AON). Here, we report the characterization and gene identification of TOO MUCH LOVE (TML), a root factor that acts during AON in a model legume Lotus japonicus. In our genetic analyses using another root-regulated hypernodulation mutant, plenty, the tml-1 plenty double mutant showed additive effects on the nodule number, whereas the tml-1 har1-7 double mutant did not, suggesting that TML and PLENTY act in different genetic pathways and that TML and HAR1 act in the same genetic pathway. The systemic suppression of nodule formation by CLE-RS1/RS2 overexpression was not observed in the tml mutant background, indicating that TML acts downstream of CLE-RS1/RS2. The tml-1 Snf2 double mutant developed an excessive number of spontaneous nodules, indicating that TML inhibits nodule organogenesis. Together with the determination of the deleted regions in tml-1/-2/-3, the fine mapping of tml-4 and the next-generation sequencing analysis, we identified a nonsense mutation in the Kelch repeat-containing F-box protein. As the gene knockdown of the candidate drastically increased the number of nodules, we concluded that it should be the causative gene. An expression analysis revealed that TML is a root-specific gene. In addition, the activity of ProTML-GUS was constitutively detected in the root tip and in the nodules/nodule primordia upon rhizobial infection. In conclusion, TML is a root factor acting at the final stage of AON.
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