The Characteristics of ABO Antibodies in Group O Thai Blood Donors

This study aimed to characterize anti‐ A and anti‐ B hemolysins, I g M, and I g G titers in T hai blood donors. Altogether, 300 serum samples from group O donors at the N ational B lood C entre, T hai R ed C ross S ociety, were screened for anti‐ A and anti‐ B hemolysins and treated with 0.01 M dithiothreitol to characterize I g M and I g G titers by standard tube technique. Antibody titers were compared with hemolysis grade. Male and female ratio = 1:1.3 and ages ranged from 17 to 60 years. The overall prevalence of anti‐ A and anti‐ B hemolysins was 69%. Anti‐ A and anti‐ B hemolysins comprised 18.3% and 16.7%, respectively and 34% had both antibodies. High titers of anti‐ A hemolysins were associated with females ( P < 0.05), and only anti‐ B I g M titers were associated with age ( P < 0.05). Interestingly, the association of anti‐ A I g M titers, anti‐ A I g G titers, and hemolysin grade was demonstrated ( P < 0.05). A significant association between hemolysin grade and anti‐ B I g M titers was found ( P < 0.05). The prevalence of anti‐ A and anti‐ B hemolysins and high titers of I g M and I g G in T hais are high. Hemolysin grade showed significant associations with I g M titers; therefore, when providing ABO ‐incompatible platelet transfusion, especially for female plateletpheresis donors, I g M high titers of anti‐ A and anti‐ B screening is suggested. J. Clin. Lab. Anal. 26:223‐226, 2012. © 2012 Wiley Periodicals, Inc.