生物
胚泡
表观遗传学
DNA甲基化
胚胎
蛋氨酸
甲基化
遗传学
胚胎发生
CpG站点
表型
男科
基因
细胞生物学
基因表达
氨基酸
医学
作者
Habib A. Shojaei Saadi,Dominic Gagné,Éric Fournier,Luis Manuel Baldoceda Baldeon,Marc‐André Sirard,Claude Robert
出处
期刊:Epigenomics
[Future Medicine]
日期:2016-08-01
卷期号:8 (8): 1039-1060
被引量:18
标识
DOI:10.2217/epi-2016-0022
摘要
There is a growing concern about the potential adverse effects of high dose folic acid (FA) supplementation before and during pregnancy. FA metabolism generates S-adenosyl methionine (SAM) which is an important cofactor of epigenetic programming. We sought to assess the impact of a large dose of SAM on early embryo development.In vitro cultured bovine embryos were treated with SAM from the eight-cell stage to the blastocyst stage. In addition to the phenotype, the genome-wide epigenetic and transcription profiles were analyzed.Treatment significantly improved embryo hatching and caused a shift in sex ratio in favor of males. SAM caused genome-wide hypermethylation mainly in exonic regions and in CpG islands. Although differentially expressed genes were associated with response to nutrients and developmental processes, no correspondence was found with the differentially methylated regions, suggesting that cellular responses to SAM treatment during early embryo development may not require DNA methylation-driven changes.Since bovine embryos were not indifferent to SAM, effects of large-dose FA supplements on early embryonic development in humans cannot be ruled out.
科研通智能强力驱动
Strongly Powered by AbleSci AI