Development of 2A Peptide-based Strategies for Constructing Multicistronic Expression Vectors

The construction of multicistronic vectors is important for biotechnology,especially for disease therapy.However,there are some limitations of the commonly used vectors such as limited capcity of vectors,imbalance of protein expression,low activity of protein or limited in the same subcellular space.2A peptide has been used for construction of multicistronic vectors in recent years.Vectors containing 2A peptide definitely outweigh other construction strategies.The resource of 2A peptide,cleave mechanism,biological character,the relationship with protein targeting and application are reviewed.