脂质体
化学
微流控
小泡
双层
纳米技术
脂质双层
生物物理学
荧光显微镜
共焦显微镜
磷脂
分散性
药物输送
肺表面活性物质
膜
荧光
材料科学
生物化学
细胞生物学
有机化学
物理
生物
量子力学
作者
Nan‐Nan Deng,Maaruthy Yelleswarapu,Wilhelm T. S. Huck
摘要
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great detail. As a result, high throughput, high-yielding routes to monodisperse liposomes with multiple compartments have not been demonstrated. Here, we report on a surfactant-assisted microfluidic route to uniform, single bilayer liposomes, ranging from 25 to 190 μm, and with or without multiple inner compartments. The key of our method is the precise control over the developing interfacial energies of complex W/O/W emulsion systems during liposome formation, which is achieved via an additional surfactant in the outer water phase. The liposomes consist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluorescence microscopy, and they can act as compartments for cell-free gene expression. The microfluidic technique can be expanded to create liposomes with a multitude of coupled compartments, opening routes to networks of multistep microreactors.
科研通智能强力驱动
Strongly Powered by AbleSci AI