Comparison of Toxic Ingredient Contents of Fermented Croton tiglium,Crude C. tiglium and Defatted C. tiglium Seed Powder

OBJECTIVE:To resolve the defects of processing attenuating the toxic effect of Croton tiglium.METHODS:By using gravimetric analysis and Coomassie brilliant blue G-250 dye binding,the fatty oil and total protein contents of two fermentation products of C.tiglium(two products fermented by Ganoderma lucidum and Beauveria bassiana were named Lingba Junzhi (LBJZ) and Baiba Junzhi (BBJZ) in Chinese),crude C.tiglium and defatted C.tiglium seed powders were determined,respectively,and GC-MS method was used to analyze the chemical constituents of the fatty oils from the four samples mentioned above.RESULTS:The toxic substances of C.tiglium,fatty oils and total proteins were greatly lowered by means of fermentation by Ganoderma lucidum and Beauveria bassiana,and even lower than defatted C.tiglium seed powders.There were 39,24,36 and 42 chemical components identified in fatty oils from crude C.tiglium,defatted C.tiglium seed powders,LBJZ and BBJZ by GC-MS analysis.Compared with crude C.tiglium,the fatty oil composition and content of the two fermented C.tiglium changed significantly,and 5 new substances emerged in LBJZ and in BBJZ,respectively.CONCLUSION:The content of toxic substances in C.tiglium reduces significantly after fermentation.The composition of C.tiglium has great changes.The trial lays the material basis for fermentation attenuating the toxic effect and maintaining therapeutic efficacy of C.tiglium.