EXTRACTION AND DETERMINATION OF URSOLIC ACID AND OLEANOLIC ACID IN KUDINGCHA

Samples of Kudingcha were extracted using methanol as solvent under ultrasonic wave. The extrace was analyzed by HPLC on a Agilent HC-C18 column (250 mm × 4.6 mm,5 μm )and detected at 215 nm, the mobile phase consisted of V(CH3OH)∶V(H2O)∶V(H3PO4) = 87∶13∶0.03 at a flow rate of 1.0 mL/min. Results show that the content of ursolic acid and oleanolic acid in Aquitoilaceae Kudingcha are high while no ursolic acid and oleanolic acid in Olcaceac Kudingcha, the content of the ursolic acid and oleanolic acid reduce with the time prolongation. This method is fast, accurate, realiable and suitable for the determination of ursolic acid and oleanolic acid in quality control of Kudingcha.