细胞内
拉曼光谱
生物量(生态学)
纳米颗粒
胶体金
表面增强拉曼光谱
化学
化学工程
纳米技术
材料科学
生物化学
拉曼散射
生物
光学
物理
工程类
农学
作者
Xiaojie Li,Xuemei Mao,Weiying Xie,Bin Liu,Feng Chen
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2022-04-05
卷期号:10 (15): 4872-4880
被引量:8
标识
DOI:10.1021/acssuschemeng.1c07432
摘要
Microalgae vary their biomass products according to the growth conditions and therefore emerge as diverse nutritional food resources. To monitor and identify the composition of algal biomass, surface-enhanced Raman spectroscopy (SERS) has been used due to the nondestructive and specific detection. However, such strategies typically require the pre-synthesis of the Raman-enhanced substrates. Herein, we proposed a streamlined method for intracellular synthesis of gold nanoparticles as a Raman substrate through biomineralization of HAuCl4 for analyzing the microalgal biomass by SERS. Our results showed that gold nanospheres (20 nm) and gold hyperbranched nanostructures (AuHNs, 120 nm) were synthesized inside Chromochloris zofingiensis through bioreduction of 0.5 and 1.0 mM HAuCl4 without any reducing and capping agents, respectively. Both the intracellular nanostructures exhibited negligible influence on the yield of biomass composition. Particularly, AuHNs showed the highest SERS activity which enhanced the Raman signal of a single C. zofingiensis cell by 9.7 times compared to the native C. zofingiensis, especially the signals for carotenoids. We further apply this intracellular biosynthesis method to other microalgae species with different cell wall structures (e.g., Euglena gracilis and Nitzschia laevis). This intracellular biosynthesis of AuHNs as Raman substrates provides a promising alternative to monitor the change of the microalgal biomass composition in a facile and time-effective way.
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