Structure-Based Affinity Maturation of Antibody Based on Double-Point Mutations

Structure-based site-directed affinity maturation of antibodies can be expanded by multiple-point mutations to obtain various mutants. However, selecting the appropriate number of promising mutants for experimental evaluation from the vast number of combinations of multiple-point mutations is challenging. In this report, we describe how to narrow candidate mutants using the so-called weak interaction analysis such as CH–π and CH–O in addition to widely recognized interactions such as hydrogen bonds.