神经炎症
大麻素
神经保护
药理学
五味子
STAT6
STAT蛋白
小胶质细胞
化学
大麻素受体2型
信号转导
受体
大麻素受体
兴奋剂
生物
转录因子
医学
炎症
内科学
车站3
生物化学
替代医学
中医药
病理
基因
作者
Jinyu Wang,Guanglin Zhang,Yunfang Yang,Xiaoying Zhang,Kaifang Shi,Xiaozhuo Zhang,Tingxu Yan,Ying Jia
标识
DOI:10.1021/acs.jafc.2c04565
摘要
Based on the current results, they showed that Schisandra chinensis lignans (SCL) ameliorated depressive-like behaviors in chronic unpredictable mild stress (CUMS) mice, alleviated neuroinflammation, and improved neuronal injury. This study aimed to explore whether SCL exerted antidepressant effects through inhibiting neuroinflammation, in turn improving neuronal injury. In vitro studies revealed that SCL blocked lipopolysaccharide-increased BV2 microglial M1 but promoted the M2 phenotype. The BV2-N2a interaction model suggested that increasing the M2 phenotype of BV2 played neuroprotective effects. The current studies demonstrated that SCL up-regulated the expression of CUMS- and LPS-decreased cannabinoid receptor type-2 (CB2R) mRNA. In vitro studies showed that the transfection of BV2 with siCrn2 blocked the SCL-increased M2 phenotype via the inactivating signal transducer and activator of transcription 6 (STAT6) pathway, further decreasing the viability of N2a cells. Finally, the possible pharmacodynamic compounds, γ-schisandrin and schisantherin A, were indicated by AutoDuck analysis. Overall, our study showed that SCL promoted microglia polarization toward the M2 phenotype, in turn exerting neuroprotective effects by activating CB2R-STAT6 signaling further to play antidepressant roles.
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