聚腺苷酸
生物
抄写(语言学)
终止因子
核糖核酸
RNA聚合酶Ⅱ
反终止
RNA聚合酶
细胞生物学
染色质
遗传学
分子生物学
发起人
DNA
基因
基因表达
语言学
哲学
作者
Jérôme O. Rouvière,Anna Salerno-Kochan,Søren Lykke‐Andersen,William A. Garland,Yuhui Dou,Om Singh Rathore,Ewa Šmidová Molska,Guifen Wu,Manfred Schmid,А. Н. Бугай,Lene Jakobsen,Kristina Žumer,Patrick Cramer,Jens S. Andersen,Elena Conti,Torben Heick Jensen
出处
期刊:Molecular Cell
[Elsevier]
日期:2023-07-01
卷期号:83 (13): 2240-2257.e6
被引量:15
标识
DOI:10.1016/j.molcel.2023.05.028
摘要
The RNA-binding ARS2 protein is centrally involved in both early RNA polymerase II (RNAPII) transcription termination and transcript decay. Despite its essential nature, the mechanisms by which ARS2 enacts these functions have remained unclear. Here, we show that a conserved basic domain of ARS2 binds a corresponding acidic-rich, short linear motif (SLiM) in the transcription restriction factor ZC3H4. This interaction recruits ZC3H4 to chromatin to elicit RNAPII termination, independent of other early termination pathways defined by the cleavage and polyadenylation (CPA) and Integrator (INT) complexes. We find that ZC3H4, in turn, forms a direct connection to the nuclear exosome targeting (NEXT) complex, hereby facilitating rapid degradation of the nascent RNA. Hence, ARS2 instructs the coupled transcription termination and degradation of the transcript onto which it is bound. This contrasts with ARS2 function at CPA-instructed termination sites where the protein exclusively partakes in RNA suppression via post-transcriptional decay.
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