腐胺
老化
体外成熟
卵母细胞
生物
男科
生发泡
细胞生物学
内分泌学
胚胎
遗传学
生物化学
医学
酶
作者
Chennan Shi,Zhengjie Yan,Yuexin Zhang,Lianju Qin,Wei Wu,Chao Gao,Li Gao,Jiayin Liu,Yugui Cui
摘要
Context Low ovarian putrescine levels and decreased peak values following luteinising hormone peaks are related to poor oocyte quantity and quality in ageing women. Aims To investigate the effects of putrescine supplementation in in vitro maturation (IVM) medium on oocyte quality and epigenetic modification. Methods Germinal vesicle oocytes retrieved from the ovaries of 8-week-old and 9-month-old mice were divided into four groups (the young, young + difluoromethylornithine (DFMO), ageing and ageing + putrescine groups) and cultured in IVM medium with or without 1 mM putrescine or DFMO for 16 h. The first polar body extrusion (PBE), cleavage and embryonic development were evaluated. Spindles, chromosomes, mitochondria and reactive oxygen species (ROS) were measured. The expression levels of SIRT1, H3K9ac, H3K9me2, H3K9me3, and 5mC levels were evaluated. Sirt1 and imprinted genes were detected. Results The PBE was higher in the ageing + putrescine group than in the ageing group. Putrescine increased the total and inner cell mass cell numbers of blastocysts in ageing oocytes. Putrescine decreased aberrant spindles and chromosome aneuploidy, increased the mitochondrial membrane potential and decreased ROS levels. Putrescine increased SIRT1 expression and attenuated the upregulation of H3K9ac levels in ageing oocytes. Putrescine did not affect 5mC, H3K9me2 or H3K9me3 levels or imprinted gene expression. Conclusions Putrescine supplementation during IVM improved the maturation and quality of ageing oocytes and promoted embryonic development by decreasing ROS generation, maintaining mitochondrial and spindle function and correcting aberrant epigenetic modification. Implications Putrescine shows application potential for human-assisted reproduction, especially for IVM of oocytes from ageing women.
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