Multifunctional Scaffold Biosensor and Drug Delivery System for Bacterial Infection Prevention During Skin Wound Healing

ABSTRACT This study investigates a multifunctional hydrogel system integrating carboxymethyl cellulose (CMC) in a 3D‐printed limonene (LIM) scaffold coated with poly(3,4‐ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS). The system allows to enhance wound healing, prevent infections, and monitor the healing progress. CMC is crosslinked with citric acid (CA) to form the hydrogel matrix (CMC‐CA), while the 3D‐printed limonene (LIM) scaffold is embedded within the hydrogel to provide mechanical support. PEDOT:PSS and curcumin‐loaded PEDOT (PEDOT:CUR) nanoparticles are integrated into the hydrogel‐membrane system for electrochemical detection of bacterial infection and controlled delivery of the antibacterial drug. The CMC‐CA hydrogel exhibits excellent mechanical properties, suitable for conforming to irregular wound surfaces. In addition to provide additional mechanical support, the LIM scaffold is used as a pillar for the incorporation of PEDOT The integration of PEDOT:PSS and PEDOT:CUR enable not only real‐time monitoring of bacterial growth but also the electrostimulated release of curcumin, which demonstrates antibacterial activity against Escherichia coli and Staphylococcus aureus . Electrostimulation of the CMC‐CA/LIM/PEDOT system promotes cell proliferation, supporting accelerated wound healing. In conclusion, the CMC‐CA/LIM/PEDOT system combines mechanical support, infection monitoring, and enhanced healing through controlled drug delivery and electrical stimulation, addressing critical challenges in wound management.