细胞毒性
酵母多糖
Wnt信号通路
阿霉素
癌症研究
细胞凋亡
化学
结直肠癌
信号转导
细胞生物学
医学
癌症
内科学
生物化学
生物
化疗
体外
作者
A. A. Rajabi,Majid Nejati,Mina Homayoonfal,Abbas Arj,Zahra Razavi,Amirreza Ostadian,Bahareh Mohammadzadeh,Massoud Vosough,Merat Karimi,Neda Rahimian,Michael R. Hamblin,Ali Arash Anoushirvani,Hamed Mirzaei
标识
DOI:10.1016/j.ijbiomac.2023.128949
摘要
Zymosan is a β-glucan isolated from Saccharomyces cerevisiae that could be employed for drug delivery. We synthesized zymosan nanoparticles and measured their structural and morphological properties using XRD, UV–Vis spectroscopy, TEM and AFM. The loading of doxorubicin (DOX) onto the nanoparticles was confirmed by FT-IR, and the DOX release was shown to be pH-dependent. The effect of these agents on C26 cell viability was evaluated by MTT tests and the expression of genes connected with the Wnt/β-catenin pathway and apoptosis were analyzed by RT-qPCR and Western blotting. Treatments were able to suppress the proliferation of C26 cells, and the zymosan nanocarriers loaded with DOX enhanced the anti-proliferative effect of DOX in a synergistic manner. Zymosan nanoparticles were able to suppress the expression of cyclin D1, VEGF, ZEB1, and Twist mRNAs. Treatment groups upregulated the expression of caspase-8, while reducing the Bax/Bcl-2 ratio, thus promoting apoptosis. In conclusion, zymosan nanoparticles as DOX nanocarriers could provide a more targeted drug delivery through pH-responsiveness, and showed synergistic cytotoxicity by modifying Wnt/β-catenin signaling and apoptosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI