Engineered thermoswitch in the baculovirus expression vector system for production of virus-like particle vaccines with minimized baculovirus contaminants

Engineered thermoswitch in the baculovirus expression vector system for production of virus-like particle vaccines with minimized baculovirus contaminants This work presents an innovative baculovirus expression system for virus-like-particle (VLP) vaccine production.A genetic thermoswitch was engineered in the baculovirus expression vector (BacFree ts ) to regulate baculovirus particle production.Temperature elevation from 27C to 33C results in a multilog reduction of co-produced baculovirus contaminants in the upstream VLP production phase.