肝细胞癌
套式聚合酶链反应
底漆(化妆品)
乙型肝炎病毒
基因
病毒学
慢性肝病
生物
聚合酶链反应
医学
病毒
遗传学
内科学
化学
肝硬化
有机化学
作者
Fedra Mokhtari,Hami Kaboosi,Seyed Reza Mohebbi,Hamid Asadzadeh Aghdaei,Mohammad Reza Zali
出处
期刊:PubMed
日期:2023-01-01
卷期号:16 (1): 520-523
摘要
Development of an amplification method for further investigation of HBV S gene variation patterns.Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC).This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region.In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated.Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing.
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