支原体
微生物学
重组DNA
生物
肿瘤坏死因子α
细胞内
免疫
分枝杆菌
巨噬细胞
拉伤
细胞内寄生虫
结核分枝杆菌
免疫系统
基因
细菌
免疫学
体外
肺结核
医学
细胞生物学
遗传学
病理
解剖
作者
Xinyan Wang,Tao Luo,Zonghai Chen,Wei Liao,Yi Wang,Lang Bao
出处
期刊:PubMed
日期:2022-09-01
卷期号:53 (5): 828-833
摘要
To investigate the effect of Mycobacterium tuberculosis ( Mtb) higBA on bacterial stress response and intracellular infection and immunity.The target gene amplified from Mtb H37Rv genome was cloned to the vector and then transferred to Mycobacterium smegmatis ( Ms) to construct a recombinant strain. Stress response experiment and Raw264.7 mouse macrophage infection was carried out with Ms_higBA, the recombinant strain, and Ms_ vec, the vector strain. Tests were conducted to measure bacterial colony forming unit (CFU) and transcriptional levels of cytokines, including interleukin ( IL)-1β, IL-6, IL-10, IL-12 p40, interferon ( IFN)- γ, tumor necrosis factor ( TNF)- α, and inducible nitric oxide synthase ( iNOS).The recombinant strain, Ms_higBA, was constructed successfully. According to the findings of the stress response experiment, higBA could indeed enhance bacterial survival under certain conditions of in vitro culture. Intracellular infection experiment demonstrated that higBA enhanced bacterial survival in macrophages and influenced the transcriptional level of cytokines.The higBA genes from Mtb play a role in bacterial stress response and intracellular infection and immunity.
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