MTT法
细胞生长
免疫印迹
癌症研究
化学
细胞培养
生长抑制
分子生物学
生物
生物化学
基因
遗传学
作者
Yiyi Ye,Xianghui Han,Baofeng Guo,Zhenping Sun,Sheng Liu
标识
DOI:10.1016/j.etap.2013.03.012
摘要
In this study, two invasive mammary carcinoma cells (MDA-MB-231 and 4T1) were utilized to evaluate the inhibitory activities of platycodin D, osthole, and the two in combination. The anti-proliferative effect was tested using the MTT and BrdU assay, and the combination of 15μM osthole and 75μM platycodin D was used for subsequent analyses. The anti-invasive effect was evaluated by the transwell assay. The results showed that the combination treatment reduced both cell proliferation and invasion. Western blot and real-time PCR revealed that the platycodin D-osthole combination significantly decreased TβRII, Smad2, Smad3 and Smad4 gene or protein expressions, as well as effectively blocked TGF-β-induced phosphorylation of Smad2 and Smad3. Thus, this study demonstrates that the anti-cancer effects of the platycodin D-osthole combination in breast cancer cells involve proliferation inhibition and invasion blockade, both of which may be mediated by perturbations in the TGF-β/Smads pathway.
科研通智能强力驱动
Strongly Powered by AbleSci AI