基因组
16S核糖体RNA
核糖体RNA
环境DNA
肠道菌群
细菌
基因组
门
微生物学
作者
Irina Kurina,Anna Popenko,Natalia Klimenko,Stanislav I. Koshechkin,Liubov Chuprikova,М. Л. Филипенко,Alexander V. Tyakht,Dmitry Alexeev
标识
DOI:10.1016/j.mcp.2020.101570
摘要
Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the "golden standard" for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks.
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