细胞毒性T细胞
生物
免疫学
免疫系统
病毒学
抗原
体外
遗传学
作者
Benjamin J Meckiff,Ciro Ramírez-Suástegui,Vicente Fajardo,Serena Chee,Anthony Kusnadi,Hayley Simon,Simon Eschweiler,Alba Grifoni,Emanuela Pelosi,Daniela Weiskopf,Alessandro Sette,Ferhat Ay,Grégory Seumois,Christian Ottensmeier,Pandurangan Vijayanand
出处
期刊:Cell
[Elsevier]
日期:2020-11-01
卷期号:183 (5): 1340-1353.e16
被引量:431
标识
DOI:10.1016/j.cell.2020.10.001
摘要
The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper cells and cytotoxic T helper (TH) cells (CD4-CTLs) responding to SARS-CoV-2 and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness, which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional TH1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities.
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