Purification and characterization of native type XIV collagen.

der Rest, M. (1991) J. Biol.Chem.266,.In the present study, we describe the purification and the characterization of the intact native form of this newly discovered collagen.By using only two chromatographic steps we were able to obtain pure type XIV collagen.Furthermore, minor modifications of the protocol allowed us to perform the simultaneous large scale purification of type XI1 and type XIV collagens from the same tissue.Intact type XIV collagen migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as two bands of 220 and 290 kDa (reducing conditions).After collagenase treatment, a single band of 190 kDa is observed, which represents the large non-collagenous domain of the molecule (NC3).Rotary shadowing electron micrographs of intact type XIV collagen show a cross-shaped structure formed by a thin tail attached through a central globule to three identical "fingers."These properties are similar to those previously described for intact chicken type XI1 collagen (Dublet, B.,