化学
白蛋白
过氧化氢异丙苯
过氧化氢
牛血清白蛋白
食腐动物
过氧化物酶
血清白蛋白
药物化学
生物化学
核化学
酶
抗氧化剂
催化作用
作者
R. Pirisino,P. Di Simplicio,G. Ignesti,G Bianchi,Pietro Barbera
出处
期刊:Pharmacological Research Communications
[Elsevier]
日期:1988-07-01
卷期号:20 (7): 545-552
被引量:51
标识
DOI:10.1016/s0031-6989(88)80081-x
摘要
The concentration, the reactivity of sulfhydryl (SH) groups and the peroxidase-like activity (PLA) of bovine serum albumin (BSA) have been determined in vitro after treatment with peroxides. Tert-butylhydroperoxide (tBOOH), cumene hydroperoxide (CuOOH), benzoyl hydroperoxide (BOOH) and hydrogen peroxide reacted with BSA, decreasing the titratable SH group concentration and increasing the value of the ratio between the reaction rate and the concentration of albumin SH groups in the sulfhydryl-disulfide exchange reaction. This value was defined as reaction constant (Kr). PLA of albumin was independent of the presence of the SH group, as SH depleted BSA maintained the same activity as the control. From our findings it derives that albumin may have two possibilities of scavenging peroxides: PLA and the SH group. The plasma SH concentration, Kr and PLA of albumin were also determined in carrageenan paw edema and in experimental adjuvant-arthritis in rats. A decrease in SH concentration, an increase in Kr and PLA of rat plasma albumin were observed in both inflammatory processes.
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