斑马鱼
鲜味
瞬时受体电位通道
品味
味觉感受器
生物
细胞生物学
受体
共域化
转导(生物物理学)
原位杂交
信号转导
分子生物学
神经科学
生物化学
基因表达
基因
作者
Yuki Yoshida,Kana Saitoh,Yasushi Aihara,Shinji Okada,Takumi Misaka,Keiko Abe
出处
期刊:Neuroreport
[Ovid Technologies (Wolters Kluwer)]
日期:2007-10-08
卷期号:18 (15): 1517-1520
被引量:27
标识
DOI:10.1097/wnr.0b013e3282ec6874
摘要
In mammals, transient receptor potential (TRP) channel M5 (TRPM5) is coexpressed with phospholipaseC-beta2 (PLC-beta2) in the taste receptor cells, and both PLC-beta2 and TRPM5 are essential elements in the signal transduction of sweet, bitter and umami stimuli. In this study, we identified the zebrafish homologue of TRPM5 (zfTRPM5) and examined its expression in the gustatory system by in-situ hybridization. Using a transgenic zebrafish line that expressed green fluorescent protein under the control of the PLC-beta2 promoter, we showed that zfTRPM5 is expressed in green fluorescent protein-labeled cells of the taste buds. These results demonstrate that zfTRPM5 and PLC-beta2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors.
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