非阻塞I/O
葡萄糖氧化酶
傅里叶变换红外光谱
检出限
过氧化物酶
卟啉
化学
核化学
纳米颗粒
纳米复合材料
基质(水族馆)
催化作用
透射电子显微镜
化学工程
组合化学
材料科学
生物传感器
光化学
纳米技术
有机化学
色谱法
酶
工程类
作者
Qingyun Liu,Yan-ting Yang,Hui Li,Renren Zhu,Qian Shao,Shanguang Yang,Jingjing Xu
标识
DOI:10.1016/j.bios.2014.08.062
摘要
NiO nanoparticles (NPs) and 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin (H2TCPP) functionalized NiO nanoparticles (H2TCPP-NiO nanocomposites) have been prepared by a facile method and characterized by powder X-ray diffraction (XRD), transmission electron microscope (TEM) and Fourier transform infrared spectra (FTIR), respectively. NiO NPs and H2TCPP-NiO nanocomposites have been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic analysis indicated that the catalytic behavior was in accord with typical Michaelis-Menten kinetics. And these nanoparticles also exhibited strong affinity for the substrates of 3,3,5,5-tetramethylbiphenyl dihydrochloride (TMB). Experimental results showed that H2TCPP-NiO NPs exhibited a high sensitivity and a low detection limit towards H2O2 (8.0 × 10(-6) M). The H2TCPP-NiO NPs/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over arrange of 0.05-0.50 mM with a limit of detection 2.0 × 10(-5)M. Fluorescence probe experiments demonstrated that the peroxidase-like activity of H2TCPP-NiO NPs originated from the generation of OH radical. Thus it may provide great potential applications in biomedicine, biotechnology and environmental chemistry.
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