融合基因
染色体易位
生物
融合蛋白
荧光原位杂交
嵌合基因
同源盒
分子生物学
融合转录本
癌症研究
白血病
断点
基因
遗传学
染色体
基因表达
重组DNA
作者
Samina Z. Raza-Egilmez,Sheila N. Jani-Sait,Mauro Grossi,Michael J. Higgins,Thomas B. Shows,Peter D. Aplan
出处
期刊:PubMed
日期:1998-10-01
卷期号:58 (19): 4269-73
被引量:52
摘要
A novel chromosomal translocation, t(2;11)(q31;p15), was identified in a patient with therapy-related acute myelogenous leukemia (t-AML). Fluorescence in situ hybridization experiments mapped the breakpoint near NUP98; Southern blot analysis demonstrated that the nucleoporin gene NUP98 was disrupted by this translocation. We used rapid amplification of cDNA ends to identify a chimeric mRNA. An in-frame, chimeric mRNA that fused NUP98 sequences to the homeobox gene HOXD13 was cloned; the predicted fusion protein contains both the GLFG repeats from NUP98 as well as the homeodomain from HOXD13. The NUP98-HOXD13 fusion is structurally similar to the NUP98-HOXA9 fusion previously identified in patients with AML, leading to the speculation that NUP98-homeobox gene fusions may be oncogenic. Moreover, this report, along with a recent study that demonstrated NUP98-DDX10 fusions in patients with t-AML, raises the possibility that NUP98 may be a previously unsuspected target for chromosomal translocations in patients with t-AML.
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