SMART (Shuimu Automated Reconstruction Technology): An Integrated Computational Platform for Streamlining Cryo-Electron Microscopy Workflow

⁺-permeable lysosomal cation channel, achieving a global resolution of 2.38 Å by gold-standard Fourier shell correlation (FSC) at the 0.143 criterion. All three modules (DataSmart, CryoSmart, and ModelSmart) were applied sequentially to the same TRPML1 dataset within this study. The protocol is designed to be accessible to users with varying levels of cryo-EM experience.