纳米医学
单克隆抗体
细胞毒性
表阿霉素
共轭体系
曲妥珠单抗
纳米颗粒
材料科学
癌细胞
结合
化学
毒品携带者
体外
癌症研究
药物输送
乳腺癌
药理学
抗体
纳米技术
癌症
医学
免疫学
生物化学
内科学
有机化学
数学分析
聚合物
数学
作者
Fatemeh Fathian kolahkaj,Katayoun Derakhshandeh,Farnaz Khaleseh,Abbas Hemmati Azandaryani,Kamran Mansouri,Mozafar Khazaei
标识
DOI:10.1016/j.jddst.2019.101136
摘要
Targeted nanomedicine plays an essential role in cancer therapy by delivering the drug to the malignant tumor cells directly. In this study, one of the potent anthracyclines, epirubicin (EPI), has been encapsulated in PLGA nanoparticles (NPs) conjugated by trastuzumab, as a monoclonal antibody (mAb). NPs was prepared by using nanoprecipitation technique. Characterizations have been performed by means of PCS, SEM, FT-IR and DSC techniques and then the optimum nanoparticles have been conjugated to mAb by an amide linkage. The results of the NPs preparation method showed the percentage yield of more than 90.55 ± 10.50% and entrapment efficiency of 91.05 ± 8.75%. PCS results showed that the average size of prepared NPs is approximately 74.66 ± 9.29 nm and 141 ± 58.41 nm for unconjugated and conjugated NPs respectively and a monodisperse distribution of them has been depicted by SEM micrograph. In vitro cellular experiments on overexpressing human epidermal growth factor receptor 2 (HER2) positive and negative cell lines demonstrated that mAb conjugated EPI-NPs has more cytotoxicity and drug uptake on over-expressed HER2 receptor cells in comparison to HER2 negative ones. The results of the current study have been demonstrated that targeted nanotechnology based formulations of EPI exhibit superior anticancer activity and have enormous potential in breast cancer (BC) treatment.
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