工作流程
计算机科学
信息学
虚拟筛选
高通量筛选
吞吐量
药物发现
鉴定(生物学)
自动化
药品
过程(计算)
医学
生物信息学
工程类
药理学
数据库
生物
操作系统
机械工程
电气工程
电信
无线
植物
作者
Paul Shinn,Lu Chen,Marc Ferrer,Zina Itkin,Carleen Klumpp‐Thomas,Crystal McKnight,Sam Michael,Tim Mierzwa,Craig J. Thomas,Kelli M. Wilson,Rajarshi Guha
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 11-35
被引量:9
标识
DOI:10.1007/978-1-4939-9089-4_2
摘要
The identification of drug combinations as alternatives to single-agent therapeutics has traditionally been a slow, largely manual process. In the last 10 years, high-throughput screening platforms have been developed that enable routine screening of thousands of drug pairs in an in vitro setting. In this chapter, we describe the workflow involved in screening a single agent versus a library of mechanistically annotated, investigation, and approved drugs using a full dose-response matrix scheme using viability as the readout. We provide details of the automation required to run the screen and the informatics required to process data from screening robot and subsequent analysis and visualization of the datasets.
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