Quantitative estimation of tannins by HPLC

A High Performance Liquid Chromatography method has been developed and quantitative estimation of tannins in Pueraria tuberosa (Fabaceae) using aqueous extract, very simple method has been performed. Nucleosil® C18 column was used with methanol (HPLC Grade) and water as mobile phase with ratio (50:50) with pH 4.5, at flow rate of 1 mL/min, UV detection performed at 270 nm. Total run time was 12 min and tannic acid was eluted at retention time of 3.1 minute. Calibration plots of tannic acid standard were linear over concentration range 0-60 μg/mL with respect to the mean area.