内化
莱茵衣藻
生物转化
银纳米粒子
生物物理学
细胞内
衣原体
周质间隙
纳米颗粒
材料科学
化学
化学工程
纳米技术
生物
生物化学
细胞
酶
突变体
工程类
基因
大肠杆菌
作者
Songshan Wang,Jitao Lv,Jingyuan Ma,Shuzhen Zhang
出处
期刊:Nanotoxicology
[Informa]
日期:2016-05-16
卷期号:10 (8): 1129-1135
被引量:71
标识
DOI:10.1080/17435390.2016.1179809
摘要
It is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0 mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48 h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as β-Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag+ ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
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