Expression of Recombinant Human Interleukin 12 in CHO Cell.

Four kinds of human IL12 expression plasmids pcDNA3/p35a, pcDNA3/p35b, pcDNA3/p40a, pcDNA3/p40b were constructed. These recombinant plasmids were cotransfected into Chinese hamster ovary cells and acquired CHO cell lines that could stably secrete IL12. The highest expressed amount of IL12 in CHO cell is 105 U/ml. The results showed that the expression of human IL12 in CHO cell was affected at several levels such as recombinant plasmid structure, DNA intergration, mRNA transcription and protein transpation etc.