Application of the Second Generation Sequencing Technology in Rice Genomics and Transcriptomics

The second generation sequencing technology made a revolutionary breakthrough in the field of sequencing technology with advantages,including high throughput,high precision and low cost.It provided a new method for the research in rice genomics.Here,we reviewed the theory and development of the second generation sequencing technology including sequencing by synthesis and sequencing by ligation,and summarized its applications in genomics,involving structural genomics and functional genomics,and transcriptomics of rice.Finally,the prospects of current sequencing technologies and their applications in rice research were also discussed.