Protection Effect of Ferulic Acid to PC12 Nerve Cells Induced by Aβ25-35

Objective: To observe the effect of Ferulic acid to the antioxidant capacity of model cell induced by Aβ25-35. To explore the effect and mechanism of angelica prevention and treatment of AD. Methods: We separately induced PC12 cells by using Aβ25-35 to against and intervened Ferulic acid with different concentration. MTT colorimetric method was used to detect and observation protection effect of Ferulic acid to cell proliferation on two kinds of AD models. Colorimetric method was used to detect the influence of Ferulic acid to activity of lactic dehydrogenase,MDA content,SOD activity and LDH content in damaged PC12 cell culture. We explored the gene expression of Apbb1 and Ache by RT-pcr method. Results: Ferulic acid increased the survival rate of damaged PC12 cells and the peak was at 2 mmol / L in Aβ25-35 models which can obviously decrease MDA content and improve SOD activity( P 0. 01). Ferulic acid can cut mRNA expression of Ache and Apbb1( P 0. 01). Conclusion: Ferulic acid can prevent PC12 cell from ability of the cell and Ache and mRNA expression of Ache and Apbb1.