甘油
发酵
甲烷
适应
微生物种群生物学
化学
食品科学
制浆造纸工业
生物技术
环境科学
植物
生物
生物化学
细菌
有机化学
工程类
遗传学
作者
Nga Thi Dinh,Kohei Hatta,Sang Hagk Kwon,Analiza P. Rollon,Kiyohiko Nakasaki
标识
DOI:10.1016/j.biombioe.2014.06.022
摘要
Granular sludge from a full-scale methane reactor treating brewery wastewater was used as a seed for the treatment of glycerol in a laboratory-scale repeated-batch methane reactor, and the change in the microbial community during the acclimation stages was examined. Two types of substrate solutions, a glucose, sodium acetate, and lactic acid mixture, as well as glycerol, were prepared and fed by mixing the two solutions to increase the ratio, in a stepwise manner, of glycerol from 0% to 100%, while keeping a loading of COD at 2.5 kg m−3 d−1 throughout the fermentation process. Vigorous methane gas production, approximately 580 dm3 m−3 d−1, was observed during the acclimation stages. Microbial analysis revealed that both bacterial and archaeal communities changed significantly; bacteria (genus Trichococcus and family Syntrophomonadaceae) became dominant rapidly after the start of acclimation, and archaea belonging to the hydrogenotrophic methanogens (genera Methanobacterium and Methanospirillum), increased gradually with the progress of acclimation.
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