离子霉素
钙调蛋白
信号转导
细胞生物学
G蛋白
化学
表皮生长因子
蛋白激酶C
受体
刺激
钙
生物
分子生物学
生物化学
内分泌学
有机化学
作者
Mitsunobu Hoshino,Shun Nakamura
标识
DOI:10.1016/s0006-291x(02)00731-3
摘要
A novel 25 K Ras-like protein, Rin, binds calmodulin in a Ca2+-dependent manner and is considered to participate in a calcium/calmodulin-mediated signaling pathway. However, little is known about Rin signaling mechanism. Here we examined the signal transduction pathway through Rin protein using pull down assay system. When we stimulated Rin-expressed Cos-7 cells with ionomycin, phorbol 12-myristate 13-acetate or epidermal growth factor (EGF), Rin protein was rapidly activated. Moreover, cells cotransfected with Rin and mSos showed a similar Rin activation profile, and Rin protein was coimmunoprecipitated with mSos protein in vivo. When cells were cotransfected with Rin and Ras-GTPase-activating proteins, basal Rin activity was decreased. Association with Rin and calmodulin was potentiated after stimulation and Rin activation was regulated by both calcium ion and calmodulin. These studies suggest that Rin may be involved in EGF receptor and mSos-mediated signaling pathway and may participate in calcium/calmodulin-mediated cellular processes.
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