外显子
融合基因
生物
RNA剪接
核糖核酸
融合蛋白
嵌合基因
分子生物学
间质细胞
选择性拼接
反式剪接
基因表达
信使核糖核酸
基因
内含子
剪接体
拼接因子
癌症研究
外显子剪接增强剂
细胞生物学
遗传学
重组DNA
作者
Hui Li,Jinglan Wang,Gil Mor,Jeffrey Sklar
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2008-09-05
卷期号:321 (5894): 1357-1361
被引量:249
标识
DOI:10.1126/science.1156725
摘要
Chromosomal rearrangements that create gene fusions are common features of human tumors. The prevailing view is that the resultant chimeric transcripts and proteins are abnormal, tumor-specific products that provide tumor cells with a growth and/or survival advantage. We show that normal endometrial stromal cells contain a specific chimeric RNA joining 5′ exons of the JAZF1 gene on chromosome 7p15 to 3′ exons of the Polycomb group gene JJAZ1/SUZ12 on chromosome 17q11 and that this RNA is translated into JAZF1-JJAZ1, a protein with anti-apoptotic activity. The JAZF1-JJAZ1 RNA appears to arise from physiologically regulated trans-splicing between precursor messenger RNAs for JAZF1 and JJAZ1 . The chimeric RNA and protein are identical to those produced from a gene fusion found in human endometrial stromal tumors. These observations suggest that certain gene fusions may be pro-neoplastic owing to constitutive expression of chimeric gene products normally generated by trans-splicing of RNAs in developing tissues.
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