化学
纳米
分析物
聚合物
纳米技术
线程(计算)
共价键
色谱法
化学工程
有机化学
材料科学
计算机科学
操作系统
工程类
作者
John J. Kasianowicz,Sarah E. Henrickson,Howard H. Weetall,Baldwin Robertson
摘要
It was recently shown that naturally occurring, genetically engineered or chemically modified channels can be used to detect analytes in solution. We demonstrate here that the overall range of analytes that can be detected by single nanometer-scale pores is expanded using a potentially simpler system. Instead of attaching recognition elements to a channel, they are covalently linked to polymers that otherwise thread through a nanometer-scale pore. Because the rate of unbound polymer entering the pore is proportional to its concentration in the bulk, the binding of analyte to the polymer alters the latter's ability to thread through the pore, and the signal that results from individual polymer translocation is unique to the polymer type; the method permits multianalyte detection and quantitation. We demonstrate here that two different proteins can be simultaneously detected with this technique.
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