微泡
MCF-7型
脂筏
乳腺癌
人体乳房
化学
癌
蛋白质组学
膜
MUC1号
医学
乳腺癌
生物化学
病理
内科学
小RNA
癌症
粘蛋白
基因
作者
Simon Staubach,Hanieh Razawi,Franz‐Georg Hanisch
出处
期刊:Proteomics
[Wiley]
日期:2009-05-01
卷期号:9 (10): 2820-2835
被引量:147
标识
DOI:10.1002/pmic.200800793
摘要
Apically expressed human MUC1 is known to become endocytosed and either to re-enter the secretory pathway for recycling to the plasma membrane or to be exported by the cells via the formation of multi-vesicular bodies and the release of exosomes. By using recombinant fusion-tagged MUC1 as a bait protein we followed an anti-myc affinity-based approach for isolating subpopulations of lipid rafts from the plasma membranes and exosomes of MCF-7 breast cancer cells. MUC1(+) lipid rafts were not only found to contain genuine raft proteins (flotillin-1, prohibitin, G protein, annexin A2), but also raft-associated proteins linking these to the cytoskeleton (ezrin/villin-2, profilin II, HSP27, gamma-actin, beta-actin) or proteins in complexes with raft proteins, including the bait protein (HSP60, HSP70). Major overlaps were revealed for the subproteomes of plasma membranous and exosomal lipid raft preparations, indicating that MUC1 is sorted into subpopulations of rafts for its trafficking via flotillin-dependent pathways and export via exosomes.
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