整体
色谱法
整体式高效液相色谱柱
气相二氧化硅
化学
烷基苯
纳米-
纳米颗粒
甲基丙烯酸酯
高效液相色谱法
聚合
材料科学
有机化学
纳米技术
聚合物
苯
催化作用
复合材料
作者
Cemil Aydoğan,İbrahim Y. Erdoğan,Ziad El Rassi
出处
期刊:Molecules
[Multidisciplinary Digital Publishing Institute]
日期:2022-04-01
卷期号:27 (7): 2306-2306
被引量:11
标识
DOI:10.3390/molecules27072306
摘要
A new feature of hydrophobic fumed silica nanoparticles (HFSNPs) when they apply to the preparation of monolithic nano-columns using narrow monolithic fused silica capillary columns (e.g., 50-µm inner diameter) was presented. The monolithic nano-columns were synthesized by an in-situ polymerization using butyl methacrylate (BMA) and ethylene dimethacrylate (EDMA) at various concentrations of AEROSIL®R972, called HFSNPs. Dimethyl formamide (DMF) and water were used as the porogenic solvents. These columns (referred to as HFSNP monoliths) were successfully characterized by using scanning electron microscopy (SEM) and reversed-phase nano-LC using alkylbenzenes and polyaromatic hydrocarbons as solute probes. The reproducibility values based on run-to-run, column-to-column and batch-to-batch were found as 2.3%, 2.48% and 2.99% (n = 3), respectively. The optimized column also indicated promising hydrophobic interactions under reversed-phase conditions, while the feasibility of the column allowed high efficiency and high throughput nano-LC separations. The potential of the final HFSNP monolith in relation to intact protein separation was successfully demonstrated using six intact proteins, including ribonuclease A, cytochrome C, carbonic anhydrase isozyme II, lysozyme, myoglobin, and α-chymotrypsinogen A in nano-LC. The results showed that HFSNP-based monolithic nanocolumns are promising materials and are powerful tools for sensitive separations.
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