Role of LncRNA CASC9 in oral carcinoma and its downstream gene expression and mechanism detected by nanomagnetic bead-based polymerase chain reaction

In view of the increasing incidence of oral carcinoma (OC), an in-depth understanding of the mechanism is required for future prevention and treatment. In modern medical research, the etiology of tumor diseases is mainly focused on gene changes, among which lncRNA is a trending topic. We speculated that lncRNA CASC9 may attribute to OC occurrence. To verify our conjecture, we extracted the total RNA of the research samples through nanomagnetic beads, detected the expression of CASC9 downstream genes miR-383-5p and KIF3B by polymerase chain reaction (PCR), and analyzed the impact of the three on OC cells and the targeting relationship. The total RNA extracted by nanomagnetic beads were found to be able to effectively improve the PCR speed, yield and the accuracy of the results, and reduce the allergic amplification results of the samples to be tested to obtain the best results. The detection result showed high CASC9 and KIF3B expression, and low miR-383-5p expression in OC cells. Inhibition of CASC9 and KIF3B and elevation of miR-383-5p can inhibit the viability of OC cells and boost apoptosis. Through dual-luciferase reporter (DLR) assay, the targeted regulation relationship between CASC9 and miR-383-5p and between miR-383-5p and KIF3B, was determined. Rescue experiments confirmed that CASC9 was able to modulate OC cell biological behaviors by targeting the miR-383-5p/KIF3B axis. Therefore, we argue that with high expression in OC, CASC9 can enhance the proliferation and migration of OC cells and inhibit the apoptosis through targeting the miR-383-5p/KIF3B axis.