凝聚
骨形态发生蛋白
骨形态发生蛋白2
体内
细胞生物学
化学
再生(生物学)
体外
生物物理学
生物医学工程
生物化学
生物
医学
基因
生物技术
作者
Eun Young Jeon,Seung‐Hoon Um,Jaeho Park,Youngmee Jung,Cheol Hong Cheon,Hojeong Jeon,Justin J. Chung
出处
期刊:Small
[Wiley]
日期:2022-05-10
卷期号:18 (24)
被引量:8
标识
DOI:10.1002/smll.202200416
摘要
Prompt and robust bone regeneration has been clinically achieved using supraphysiological doses of bone morphogenetic protein-2 (BMP-2) to overcome the short half-life and rapid clearance. However, uncontrolled burst release of exogenous BMP-2 causes severe complications such as heterotopic ossification and soft tissue inflammation. Therefore, numerous researches have focused on developing a new BMP-2 delivery system for a sustained release profile by immobilizing BMP-2 in various polymeric vehicles. Herein, to avoid denaturation of BMP-2 and enhance therapeutic action via localized delivery, a complex coacervate consisting of fucoidan, a marine-derived glycosaminoglycan, and poly-l-lysine (PLL) is fabricated. Superior BMP-2 binding ability and electrostatic interaction-driven engulfment enable facile and highly efficient microencapsulation of BMP-2. The microencapsulation ability of the coacervate significantly improves BMP-2 bioactivity and provides protection against antagonist and proteolysis, while allowing prolonged release. Moreover, BMP-2 containing coacervate is coated on conventional collagen sponges. The bioactivity and localized bone regenerating ability are confirmed through in vitro (human-derived stem cells), and in vivo (calvarial bone defect model) evaluations.
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