微流控
过滤(数学)
堵塞
吞吐量
膜
材料科学
微型泵
全血
微滤
微型多孔材料
生物医学工程
微流控芯片
纳米技术
色谱法
化学
计算机科学
工程类
复合材料
外科
医学
历史
统计
电信
生物化学
考古
无线
数学
作者
Yinuo Cheng,Xiongying Ye,Zengshuai Ma,Shuai Xie,Wenhui Wang
出处
期刊:Biomicrofluidics
[American Institute of Physics]
日期:2016-01-01
卷期号:10 (1)
被引量:66
摘要
Rapid separation of white blood cells from whole blood sample is often required for their subsequent analyses of functions and phenotypes, and many advances have been made in this field. However, most current microfiltration-based cell separation microfluidic chips still suffer from low-throughput and membrane clogging. This paper reports on a high-throughput and clogging-free microfluidic filtration platform, which features with an integrated bidirectional micropump and commercially available polycarbonate microporous membranes. The integrated bidirectional micropump enables the fluid to flush micropores back and forth, effectively avoiding membrane clogging. The microporous membrane allows red blood cells passing through high-density pores in a cross-flow mixed with dead-end filtration mode. All the separation processes, including blood and buffer loading, separation, and sample collection, are automatically controlled for easy operation and high throughput. Both microbead mixture and undiluted whole blood sample are separated by the platform effectively. In particular, for white blood cell separation, the chip recovered 72.1% white blood cells with an over 232-fold enrichment ratio at a throughput as high as 37.5 μl/min. This high-throughput, clogging-free, and highly integrated platform holds great promise for point-of-care blood pretreatment, analysis, and diagnosis applications.
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